Stabilized fibroblast growth factor 2 is a revolutionary anti-aging ingredient that solves the instability of growth factors in cosmetics
WHAT DOES IT DO?
- Anti-aging ingredient
- Retains skin hydration
- Enhances wrinkle reduction
- Enables skin softening
- No stabilizing additives
- Normalizes skin keratinization
- Cost-effective
- Dermatologically and clinically tested
COMPETITIVE ADVANTAGE
Native growth factors suffer from low stability limiting their utilization in cosmetics during production, transport and application. FGF2-STAB outperforms native forms of growth factors due to its significantly improved stability
FGF2-STAB® BENEFITS
01
FGF2-STAB® vs. FGF2
| FGF2 | FGF2-STAB® | FGF2-STAB® BENEFITS | |
| ACTIVITY AT 37°C | < 10 hours | > 20 days | More stable during biological processes |
| STABILITY AT 25°C | < 1 month | 2 years | Stable for longer period (transportation and storage) |
| ACTIVITY (ED50) | 5 ng/ml | 1.1 ng/ml | Lower concentration required (reduced costs) |
| MELTING TEMP. | 54 °C | 73 °C | Stable at higher temperature (heating during production) |
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Higher stability of FGF2-STAB®
SPECIFICATIONS
| INCI | AQUA SODIUM CHLORIDE DIPOTASSIUM PHOSPHATE MONOPOTASSIUM PHOSPHATE SH-POLYPEPTIDE-1 |
| CAS NUMBER | 106096-93-9 |
| APPEARANCE | Colourless aqueous solution |
| BUFFER | 250 mM Sodium chloride, 16.4 mM Potassium hydrogen phosphate, 3.6 mM Potassium dihydrogen phosphate, pH 7.5 |
| PH FOR FORMULATIONS | 5.5–7.5 (for the final cosmetic formulation) |
| CONCENTRATION (RECOMMENDED) | 1–10 ppm (1–10 μg/ml) in the final formulation |
| USE | Gels, emulsions, serums, mask |
ENDORSEMENT
“Using proteins and peptides as active ingredients is one of the latest trends in cosmetics. Substances like growth factors have a high potential of optimizing processes in various skin layers. FGF2-STAB has a clear advantage in making the skin smoother and even-coloured. Another important aspect of FGF2-STAB is reduction of transepidermal water loss which retains skin hydration. This increases its potential to be used in clients with chronically dry skin and with some skin disorders such as psoriasis or eczema. Main customers will be recruited from people with loss of skin elasticity and onset of wrinkles, including the mimic ones. FGF2-STAB can be recommended as a preventative care for physiologically aged skin.
– Assoc. Prof. Jarmila Rulcova, MD, dermatovenerologist, Ambulance of Esthetic Dermatology – SynCare
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Q&A
FGF2-STAB® is a stabilized variant of native fibroblast growth factor 2 (FGF2). FGF2 is an active molecule with essential signalling role in human cells. The mechanism of action of FGF2-STAB® is exactly the same as the native FGF2 and it retains the full biological activity.
AQUA
SODIUM CHLORIDE
DIPOTASSIUM PHOSPHATE
MONOPOTASSIUM PHOSPHATE
SH-POLYPEPTIDE-1
Native FGF2, often referred as the Wild-Type (WT), suffers of very low stability. Its biological half-life at 37°C is just 9 hours and its concentration at room temperature drops below 30% in less than 2 weeks. Therefore, native FGF2 is useless as a cosmetic ingredient and all cosmetic products based on native FGF2 contain literally a “dead” molecule.
FGF2-STAB® is stabilized by protein engineering directly in the protein structure. In particular, specific amino acids within the protein sequence were replaced with residues increasing the melting temperature. The higher melting temperature results in the higher stability of FGF2-STAB®.
FGF2-STAB® is produced as a liquid solution. Alternatively, a lyophilized powder might be shipped for an extra charge.
Native FGF2 (wild-type) is already established in the cosmetic market (INCI: SH-POLYPEPTIDE-1) and is approved as a pharmaceutical drug for wound healing in Japan. We did cytotoxicity testing of FGF2-STAB® in a huge excess with no cytotoxicity observation. In contrast to native FGF2, we only improved the stability of the protein, we did not alter the biological activity or mechanism of action. Therefore, in terms of mechanism of action, FGF2-STAB® is the same molecule as native FGF2.
No, we do not use any preservatives. FGF2-STAB® contains only the protein itself dissolved in the physiological solution.
FGF2 (also known as basic FGF – bFGF) is one of the most versatile growth factors in humans. It is necessary not just for the creation of the new tissues during embryogenesis but it plays a crucial role when the tissue is damaged (wound-healing). Unlike other ingredients having only supporting role in anti-aging, both FGF2 and FGF2-STAB® have direct biological functions in the tissue reepithelization and remodelling, fibroblast migration and collagen maturation. It is an active, signalling molecule.
Yes, we have conducted a clinical trial of FGF2-STAB® in a very simple cream formulation to avoid any efficacy shielding by other cosmetic ingredient. The efficacy study showed that FGF2-STAB® reduces wrinkles, retains skin hydration and normalizes the skin surface. Moreover, there are plenty of pre-clinical and clinical wound-healing and tissued re-modelling data in literature for FGF2. These biological functions of FGF2 are adequately important for cosmetics as well.
FGF2-STAB® is a recombinant protein produced by microbial fermentation (recombinant expression in bacteria). In short, we cultivate bacterial cells producing FGF2-STAB® in fermenter, then we break bacterial cells and isolate FGF2-STAB® by series of purification steps. This production type is referred as biotechnology-derived in pharma industry.
FGF2-STAB® is not synthetic; it is produced by microbial fermentation in bacteria. We consider it as a natural product.
FGF2-STAB® is a recombinant protein expressed in genetically modified strain of E. coli. However, FGF2-STAB® comes as a purified protein with no residual GMO content (validated by residual DNA analysis).
The penetration efficacy is 0.6 % (measure by Franz cells using Start-M membrane). That is comparable to similar product with high molecular weight. However, we believe that Franz cells is not an adequate method for detecting large and active molecules, since they might be delivered through the skin via an active transport. Importantly, we are currently working on the next generation FGF2-STAB® encapsulated in liposomes that supposed to significantly increase the penetration efficacy.
Definitely. We have conducted a robust compatibility testing of FGF2-STAB® with common cosmetic ingredients (see the technical documentation).
Of course, FGF2-STAB® can be used in creams, serums, gels or even in mask or other matrices.
This concentration range is based on the in vivo efficacy screening and in vitro cell growth analysis. It is the minimal concentration where the biological effect is observed.
The shelf-life of the aqueous solution of FGF2-STAB® is 2 years at room temperature. You can store FGF2-STAB® in refrigerator for even longer stability.
It strongly depends on the quantity. Regular orders are shipped within 25 days.