Do you need to engineer your protein of interest? Do you wish to employ user-friendly one-stop-shop software tools for the design of mutations and libraries, but do not want to spend time on tedious installations or with the Unix command-line? This interactive course will provide the essential theory and hands-on experience on a number of web-based tools suitable for mining new enzymes, engineering the activity, specificity, enantioselectivity, stability, and solubility of enzymes.


You will have the chance to learn and discuss with the developers of these tools the optimal ways to set-up calculations and adequately interpret the results. At the end of this 3-day course, you will be able to design different properties of proteins for your academic research or commercial applications on your own.

Web-tools covered: EnzymeMinerHotSpot WizardFireProtFireProtASRFireProtDBCalFitterCaver WebCaverDockLoopGrafterSolubis and SoluProt. Our web tools integrate individual software, and altogether, you will get automated access to more than 50 computational methods and 15 databases.


Keynote lecture Computational enzyme design
Theory Mining and analysis of novel enzymes
        Hands-on Identification of novel enzymes
        Hands-on Homology modelling and design of libraries
Theory Engineering enzyme activity and specificity
        Hands-on Modelling protein-ligand complexes
        Hands-on Identification of enzyme tunnels
        Hands-on Analysis of ligand pathways
        Hands-on Rational loop grafting
Theory Engineering protein stability
        Hands-on Design of stable proteins
        Hands-on Analysis of stability data
Theory Engineering protein solubility
        Hands-on Design of soluble proteins


The online workshop with be run live during 3 days (May 30–June 1, 2022), from 9 am to 6:00 pm (CET). The program will be composed of a main scientific part, with theoretical lectures and hands-on sessions. However, it will also include discussion rooms reserved for networking, exchange of experiences, contacts and cultural backgrounds. The latter will be promoted by a devoted platform.


The 3rd edition of this course is taking place online for safety reasons.


The registrations will be open between 15 March and 30 April, 2022. Early-bird discounts apply. The invoice will be issued based on the information provided during the registration. The course is limited to 50 participants, prioritized based on their registration order. Do not miss your chance and register now!


Till April 15th, 2022 650 EUR
Till April 30th, 2022 850 EUR

The registration fee includes:

  • Attendance to all theoretical lectures and hands-on sessions
  • Course materials: slides of the theoretical lectures, step-by-step protocols, useful tips & tricks, relevant literature
  • Advanced hands-on exercises
  • Trouble-shooting guidance
  • Graduation certificate