Based on fibroblast growth factor 2 (FGF2, bFGF)

  • ability to induce cell proliferation and maintain cells in their undifferentiated state
  • essential component in both stem cell culture and cultured meat media
  • intrinsically unstable and therefore requires to be added to the media often and in high concentrations

FGF2-STAB® MEAT

  • Engineered protein with improved stability and longevity
  • Patented molecule (WO2017089016A1)
  • Called FGF2-G3 in publications
  • Suitable for cultured meat applications – possibility of reformulation according to the customer’s specs
  • Option of supply/collaboration/license agreements

COMPETITIVE ADVANTAGE

  • Much lower dosage needed (up to 20-times)
  • Longer half-life (50-times)
  • Fully retained biological activity
  • No need for stabilizing additives
  • Animal-free product
FGF10-STAB

01

50-times longer half-life

Measured by activation of ERK in human ESC CCTL14 culture

02

Temperature stability enhanced by 19°C

Measured by circular dichroism spectroscopy

 

03

Lower dosage required*

Measured by NIH/3T3 fibroblast cell proliferation

* Up to 20-times lower dosage required if used in B8 media formulation (Kuo et al., 2020)

 

04

Enhanced stability in cell proliferation assay

Tested in BAF3 cells expressing FGFR2c receptor

VISION

  • FGF2-STAB® Meat is ready to become a game changer in the cultured meat space as a highly effective and cost-efficient product with global traction
  • FGF2 is a key component of cultured meat media representing around 60% of media cost
  • Scale-up of cultured meat production will require significant reduction of media cost

 

Possibility of reformulation according to customers’ specs and regulations

Open to agreements for supply / collaborations / licenses

 

Literature

Dvorak P, Bednar D, Vanacek P, et al. Computer-assisted engineering of hyperstable fibroblast growth factor 2. Biotechnol Bioeng. 2018;115(4):850-862. doi:10.1002/bit.26531

Koledova Z, Sumbal J, Rabata A, et al. Fibroblast Growth Factor 2 Protein Stability Provides Decreased Dependence on Heparin for Induction of FGFR Signaling and Alters ERK Signaling Dynamics. Front Cell Dev Biol. 2019;7:331. Published 2019 Dec 12. doi:10.3389/fcell.2019.00331

Kuo HH, Gao X, DeKeyser JM, et al. Negligible-Cost and Weekend-Free Chemically Defined Human iPSC CultureStem Cell Reports. 2020;14(2):256-270. doi:10.1016/j.stemcr.2019.12.007